传染性支气管炎病毒S1和N基因遗传变异频率的分析

doi:10.11843/j.issn.0366-6964.2015.06.015

Abstract

Abstract:

This study was undertaken to investigate the prevalence and molecular variation of infectious bronchitis virus in the northern area of China.Thirteen infectious bronchitis viruses (IBV) strains obtained from commercial chickens in northern China between 2012 and 2013 were isolated and identified by the sequencing of the entire S1 gene.The variants were distinguished by phylogenetic analysis and alignment in comparison with the vaccination-challenge tests that were performed using heterogeneous strains.The results showed that both the S1 and N gene of the prevailing IBV shared higher homologies.Furthermore，S1 gene shared nucleotide (amino acid) homology of 94.0%-100% (93.1%-100%)，and N gene had nucleotide (amino acid) 98.4%-99.1% homology (98.1%-99.8%)，receptively.Compared with the classical vaccine strain，there were at least three hyper-variable regions were found in S1 gene，and point mutations，deletions and insertions of IBV strains were also occurred at the genomic level，which were concentrated in the 53-150，and 250-290.Four amino acids were found missing in GM/13 at 62-65.Further analysis showed that the IBV epidemic strains shared highest homology （94.9%-97.2%） with A2 strains and the nucleotide (nt) mutation frequency averaged 2.4 nt×10^-3/year in view of S1 gene；while，the N gene was the LX4 strains of the homologous highest (93.0%-93.5%)，the average annual variation in frequency 5.1 nt×10^-3/year.Those findings suggest that the recent IBV epidemic strains may be recombinant of A2 and LX4 type strains，and N gene mutation frequency is greater than of the S1 gene.

CLC Number:

S852.659.6

SHENG Xiao-dan，XU Huai-ying，LIU Xia，HUANG Di-hai，QU Xin-ze，QIN Zhuo-ming. Genetic Variation Frequency of S1 and N Gene of Infectious Bronchitis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 989-997.

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Genetic Variation Frequency of S1 and N Gene of Infectious Bronchitis Virus

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